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The membranes were blocked using 3% BSA (bovine serum albumin) to prevent non-specific interactions and were incubated overnight with the primary antibodies (anti-BDNF, Affinity Biosciences Co.) and (anti-CREB, Affinity Biosciences Co.) for at least sixty minutes

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The SURPASS and SURMOUNT trial programs Tirzepatide was evaluated in over 10,000 participants across the SURPASS (type 2 diabetes) and SURMOUNT (obesity) trial programs